Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c4ra01027b Click here for additional data file.

نویسندگان

  • Anika Raulf
  • Christoph K. Spahn
  • Patrick J. M. Zessin
  • Kieran Finan
  • Stefan Bernhardt
  • Alexander Heckel
  • Mike Heilemann
چکیده

We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.

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منابع مشابه

iPAINT: a general approach tailored to image the topology of interfaces with nanometer resolution† †Electronic supplementary information (ESI) available: Fig. S1–S8. See DOI: 10.1039/c6nr00445h Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.

Understanding interfacial phenomena in soft materials such as wetting, colloidal stability, coalescence, and friction warrants non-invasive imaging with nanometer resolution. Super-resolution microscopy has emerged as an attractive method to visualize nanostructures labeled covalently with fluorescent tags, but this is not amenable to all interfaces. Inspired by PAINT we developed a simple and ...

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عنوان ژورنال:

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2014